OS-FRET: A New One-Sample Method for Improved FRET Measurements
نویسندگان
چکیده
منابع مشابه
Preparing sample chambers for single-molecule FRET.
Single-molecule (sm) fluorescence detection is a powerful method for studying biological events without time and population averaging. Förster (fluorescence) resonance energy transfer (FRET) is a spectroscopic technique in which the efficiency of energy transfer from donor to acceptor molecules is used to determine distances between molecules in the 30-80 Å range. Structural changes in biologic...
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Fluorescence resonance energy transfer (FRET) is a technique used to measure the interaction between two molecules labeled with two different fluorophores (the donor and the acceptor) by the transfer of energy from the excited donor to the acceptor. In biological applications, this technique has become popular to qualitatively map protein-protein interactions, and in biophysical projects it is ...
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This article supplies raw data related to a research article entitled "Joint refinement of FRET measurements using spectroscopic and computational tools" (Kyrychenko et al., 2017) [1], in which we demonstrate the use of molecular dynamics simulations to estimate FRET orientational factors in a benchmark donor-linker-acceptor system of enhanced cyan (ECFP) and enhanced yellow (EYFP) fluorescent ...
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Sensitive methods developed to measure laser-induced fluorescence from trapped ions have been applied to study the intermediate states of the dissociation of oligonucleotide duplexes. Double-stranded oligonucleotide anions were prepared with FRET donor/acceptor pairs attached. Gas phase ions were generated by electrospray ionization and injected into a heated quadrupole ion trap where they were...
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In this issue of the Biophysical Journal, Jarecki et al. (1) investigate the possibility of combining two strategies commonly used to measure distances between loci in a number of systems. The first strategy, Förster resonance energy transfer (FRET), is a spectroscopic method based on energy transfer between two fluorescent molecules: upon transferring its excitation energy to an acceptor via a...
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ژورنال
عنوان ژورنال: Biochemistry
سال: 2011
ISSN: 0006-2960,1520-4995
DOI: 10.1021/bi101188b